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| #6340413 in Books | Springer | 2000-12-31 | Original language:English | PDF # 1 | 9.21 x1.38 x6.14l,2.30 | File type: PDF | 606 pages | |
It is thirty years since the technique of high-yield preparation of isolated hepatocytes, by collagenase perfusion of the liver, was published. The original method described by Berry and Friend has undergone many minor modifications by other workers, and the two-step procedure introduced by Seglen in 1976 has become the most frequent way to prepare hepatocyte suspensions. An important development introduced by Bissell in 1973 was the use of the cells as the first step in...
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